US Patent:
20140227783, Aug 14, 2014
Inventors:
Evan Masataka Masutani - Honolulu HI, US
Brandon Aran Yoza - Honolulu HI, US
Travis Tadashi Tanaka - Kailua HI, US
Christopher Randall Russ - Hampton VA, US
Andrew Kevin Donald Ellison - Honolulu HI, US
Dominic Seth Reiss - Honolulu HI, US
International Classification:
B01J 19/12
C12N 5/00
US Classification:
435397, 1061601, 1061461, 4221863
Abstract:
A novel method for building complex three-dimensional scaffolds for biomimetic applications such as in vitro organ growth, using a gelatin/sugar/water gel, ultraviolet radiation, and heat or enzyme is described. The method produces gelatin-sugar hydrogels demonstrating greater thermal stability, mechanical strength, and resistance to enzymatic degradation. The invention also provides a means to assemble the gelatin sugar hydrogel films into complex three-dimensional structures (scaffolds). To account for the native biochemical factors present in natural scaffolds, methods of conjugating such factors to the gelatin-sugar hydrogel are described. These scaffolds can then be applied for tissue culturing and organ growth. The present invention also describes a system and apparatus for constructing these complex three-dimensional scaffolds by taking advantage of the physical and chemical properties of the gelatin-sugar hydrogel.
