Inventors:
Arun Srivastava - Indianapolis IN, US
Keyun Qing - Indianapolis IN, US
Xu-Shan Wang - Carmel IN, US
Selvarangan Ponnazhagan - Birmingham AL, US
Anil Bajpai - Indianapolis IN, US
Assignee:
Advanced Research & Technology Institute - Bloomington IN
International Classification:
A61K 48/00
A61K 39/395
C12N 15/864
C12N 15/63
C07H 21/04
US Classification:
4241351, 424 931, 424 932, 424 936, 4241301, 4241341, 4241411, 4241521, 435 5, 435 6, 435 71, 435455, 435456, 435457, 435325, 435366, 4353201, 514 2, 514 44, 536 231, 536 235
Abstract:
The present invention identifies a protein, designated the D-sequence-binding protein (D-BP), is phosphorylated at tyrosine residues and blocks AAV-mediated transgene expression in infected cells by inhibiting the leading strand viral DNA synthesis. More particularly, the present invention demonstrates that D-BP is phosphorylated by EGF-R protein tyrosine kinase. Methods of increasing transcription and promoting replication of transgenes exploiting this information are disclosed herein.