Resumes
Resumes
Sankha Bhowmick Phones & Addresses
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106 Burt St, Norton, MA 02766 (508) 285-3988
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2033 Knapp St, Saint Paul, MN 55108 (651) 917-0659
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Devon, PA
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Mansfield, MA
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Cambridge, MA
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Minneapolis, MN
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Quincy, MA
Work
Position:
Clerical/White Collar
Publications
Us Patents
Systems And Methods For Cell Preservation
View pageUS Patent:
20050277107, Dec 15, 2005
Filed:
Jan 13, 2005
Appl. No.:
11/035419
Inventors:
Mehmet Toner - Wellesley MA, US
Jason Acker - Spruce Grove, CA
Tani Chen - Acton MA, US
Alex Fowler - South Dartmouth MA, US
John Baust - Vestal NY, US
Sankha Bhowmick - Norton MA, US
Jason Acker - Spruce Grove, CA
Tani Chen - Acton MA, US
Alex Fowler - South Dartmouth MA, US
John Baust - Vestal NY, US
Sankha Bhowmick - Norton MA, US
International Classification:
A01N001/02
US Classification:
435002000
Abstract:
The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and/or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and/or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and/or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and/or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.