Inventors:
Richard E. Pelc - Libertyville IL
Nicholas S. Chibucos - Bloomingdale IL
Roeland F. Papen - Guilford CT
Wilhelm Meyer - Tostedt, DE
Assignee:
Packard Instrument Company, Inc. - Downers Grove IL
International Classification:
B01L 302
US Classification:
422100, 7386411, 222 57, 222333, 436 49, 436 54, 436180
Abstract:
A microvolume liquid handling system includes a microdispenser employing a piezoelectric transducer attached to a glass capillary, a positive displacement pump for priming and aspirating transfer liquid into the microdispenser, controlling the pressure of the liquid system, and washing the microdispenser between liquid transfers, and a pressure sensor to measure the liquid system pressure and produce a corresponding electrical signal. Dispensing of a single sub-nanoliter drop can be detected in real time. As the result of dispensing the liquid in sub-nanoliter droplets, the dispensed volume can be precisely controlled. The dispenser automatically detects the liquid surface of the transfer liquid, automatically aspirate, analyze desired volume of the transfer liquid, dispense the transfer liquid without contacting the destination vessel or its contents, and automatically wash off the transfer liquid from dispensing system after each transfer. This system is capable of automatically sensing liquid surfaces, aspirating liquid to be transferred, and then dispensing small quantities of liquid with high accuracy, speed and precision. The system is pulsated at high frequency to prevent or eliminate clogging.
