Mohanram Sivaraja

6569619, May 27, 2003

Jul 29, 1998

09/124312

Mohanram Sivaraja - Palo Alto CA

Tularik, Inc. - South San Francisco CA

C12G 168

435 6, 435 71, 435 18, 435195

The present invention provides solid phase assays for measuring helicase activity in the presence or absence of a potential helicase activity modulator. High throughput methods, compositions, kits and integrated systems are provided for detecting helicase activity in vitro and for measuring the effect of potential helicase activity modulators.

60430389, Mar 28, 2000

Mar 31, 1998

9/052924

Mohanram Sivaraja - Palo Alto CA
Ashok Sanadi - Sunnyvale CA
M. Gregory Peterson - Millbrae CA

Tularik, Inc. - South San Francisco CA

C12Q 168
C12P 1934
C07H 2102
C07H 2104

435 6

This invention provides novel assays for primase activity and for identifying modulators of primase activity. The assays include both solid-phase and liquid-phase methods that are amenable to high throughput screening methods. The assays of the invention are readily adaptable to numerous types of primase, and are capable of identifying novel classes of primase activity modulators.

57053448, Jan 6, 1998

Mar 14, 1996

8/616046

Heidi Giordano - Oakland CA
Michael G. Peterson - Millbrae CA
Mohanram Sivaraja - Mountain View CA

Tularik, Inc. - South San Francisco CA

C12Q 168

435 6

The invention provides screening assays for identifying an inhibitor of a pathogenic helicase activity, e. g. a helicase derived from a pathogenic infectious organism such as a bacterium, protozoan or fungus. Helicase activity is detected by the solid-phase, preferential capture of retained (non-liberated) single-stranded nucleic acid comprising a detectable label.

6153384, Nov 28, 2000

Feb 19, 1998

9/025979

Anthony Simon Lynch - Pacifica CA
Ashok Ramesh Sanadi - Sunnyvale CA
Mohanram Sivaraja - Palo Alto CA

Tularik Inc. - South San Francisco CA

C12Q 168
C12P 1934

435 6

Screening assays for identifying ligase activity modulators are provided, in both solid phase and liquid phase formats. Solid phase formats detect ligase activity by ligating a labelled nucleic acid to a capture nucleic acid in the presence of the ligase modulator and detection of the labelled nucleic acid. Liquid phase assays detect ligation-dependent changes in interactive labels between nucleic acids such as proximity quenching of fluorescent labels. Compositions, apparatus and integrated systems for assays are also provided.

61839566, Feb 6, 2001

Mar 31, 1998

9/052995

Mohanram Sivaraja - Palo Alto CA
Berta Strulovici - Lower Gwynedd PA
Osvaldo A. Flores - Redwood City CA

Tularik, Incorporated - So. San Francisco CA

C12Q 168

435 6

High-throughput in vitro assays are provided for identifying modulators of transcription activity and RNA expression. New assays, related compositions, apparatus and integrated systems are provided.