Michael G Conrad

6635435, Oct 21, 2003

Jul 9, 1999

09/350461

Michael J. Conrad - Escondido CA
Liyan He - Escondido CA

Chromagen, Inc. - San Diego CA

C12Q 134

435 18, 435 23, 536 41

The subject invention provides compounds useful as fluorogenic substrates. Upon hydrolysis of a hydrolyzable group, a halo-pyrene substituted molecule is developed which is highly fluorescent, water soluble and exhibits several desirable characteristics, including a large Stokers shift.

6654119, Nov 25, 2003

Apr 20, 2000

09/552803

Gene Gould - Cardiff CA
Michael J. Conrad - Escondido CA

Chromagen, Inc. - San Diego CA

G01N 2164

356318, 356333, 2504581

A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.

6949632, Sep 27, 2005

Aug 27, 2002

10/229628

Michael J. Conrad - Escondido CA, US
Liyan He - Escondido CA, US

Chromagen, Inc. - Escondido CA

C07G011/00

536 41, 435 18, 435 23

The subject invention provides compounds useful as fluorogenic substrates for the hydrolytic enzymes. Upon hydrolysis of the hydrolyzable group, a halo-pyrene substituted molecule is developed which is highly fluorescent, water soluble and exhibits several desirable characteristics, including a large Stokes' shift.

7317111, Jan 8, 2008

Sep 23, 2003

10/669584

Ram Bhatt - San Diego CA, US
Michael J. Conrad - Escondido CA, US
Azzouz Bencheikh - San Diego CA, US
Yifeng Xiong - San Diego CA, US

Aries Associates, Inc. - San Diego GA

C07C 309/50
C07C 331/24
C07D 207/404
G01N 21/00

548528, 558 17, 562 52, 562 55, 436172, 436800

The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

7352459, Apr 1, 2008

Dec 31, 2001

10/039769

Gene Gould - Cardiff CA, US
Michael J. Conrad - Escondido CA, US

Chromagen, Inc. - Escondido CA

G01J 3/18
G01N 21/64

356318, 356333, 2504581

A fluorescence spectrophotometer system may be implemented in scanning fluorescence polarization detection applications. A wavelength and area scanning fluorescence spectrophotometer system may include a light source, an excitation double monochromator, an excitation/emission light transfer module, an emission double monochromator, a high speed timer-counter circuit board, a precision positioning apparatus for positioning a sample relative to the focal plane of the excitation light, and polarizing filters at the excitation side and the emission side. The system may be operative to analyze more than one fluorescent compound in the sample; additionally or alternatively, the system enables analysis of samples from selected ones of a plurality of samples.

7411673, Aug 12, 2008

Sep 8, 2003

10/658363

Gene Gould - Cardiff CA, US
Michael J. Conrad - Escondido CA, US

Chromagen, Inc. - San Diego CA

G01N 21/64

356317, 2504581, 356318

A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.

7667048, Feb 23, 2010

Dec 29, 2007

11/967134

Ram Bhatt - San Diego CA, US
Michael J. Conrad - Escondido CA, US
Azzouz Bencheikh - Rancho Santa Fe CA, US
Yifeng Xiong - San Diego CA, US

Aries Associates, Inc. - Escondido CA

C07C 309/50
C07C 309/51
C07C 311/29
C07C 331/20
C07D 207/404

548528, 558 17, 562 52, 562 55, 436172, 436800

The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

20080225289, Sep 18, 2008

Mar 31, 2008

12/060170

Gene Gould - Cardiff CA, US
Michael J. Conrad - Escondido CA, US

CHROMAGEN, INC. - San Diego CA

G01J 3/40

356303

A fluorescence spectrophotometer system may be implemented in scanning fluorescence polarization detection applications. A wavelength and area scanning fluorescence spectrophotometer system may include a light source, an excitation double monochromator, an excitation/emission light transfer module, an emission double monochromator, a high speed timer-counter circuit board, a precision positioning apparatus for positioning a sample relative to the focal plane of the excitation light, and polarizing filters at the excitation side and the emission side. The system may be operative to analyze more than one fluorescent compound in the sample; additionally or alternatively, the system enables analysis of samples from selected ones of a plurality of samples.