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Janine G Borgaro

from Winchester, MA
Age ~41
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Also known as:
Janine C Borgaro, Janine Graham
Related to:
David A Ertrachter, 70Janine Patricia Graham, 63
Connected to:
Raoul F Borgatti, 109Adolfo A Borge, 46Angela L Borge, 90Armando Borge, 44Calvin D Borge, 60Cesar A Borge, 47Christina M Borge, 58David A Borge, 60Gary J Borge, 45Herlinda B Borge, 63

Janine Borgaro Phones & Addresses

  • Winchester, MA
  • 13 Greene St, North Reading, MA 01864
  • Beverly, MA
  • Yorktown Heights, NY
  • Selden, NY
  • Mahopac, NY
  • 25 Trask Ct APT 32, Beverly, MA 01915

Work

Company: New england biolabs 2011 Position: Postdoctoral research fellow

Education

School / High School: Stony Brook University 2005 Specialities: Doctor of Philosophy in Chemistry

Skills

Molecular Biology: Performed plasmid con... • cloning recombinant DNA • site-directed mutagenesis • RT-PCR and inverse PCR Recombinant. Prot... • protein expression and purification • affinity chromatography • gel filtration • ion exchange • FPLC • AKTA • dialysis • SDS or Native PAGE and western blotting.... • Utilized fluorescence spectroscopy • UV-spectroscopy • Circular Dichroism (CD) • Biacore • HPLC • ESI-MS • and LC-MS. Radiochemistry: Designed and ... • 14C • 3H • Quantitated radioactivity in instruments... • purification and characterization of enz... • Grafit it (Data processing) • ChemDraw • CorelDraw.

Industries

Biotechnology

Resumes

Resumes

Janine Borgaro Photo 1

Applications And Product Development Scientist Ii

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Location:
13 Greene St, North Reading, MA 01864
Industry:
Biotechnology
Work:
New England Biolabs Nov 2011 - Feb 2014
Post Doctoral Fellow
New England Biolabs Nov 2011 - Feb 2014
Applications and Product Development Scientist Ii
Stony Brook University Aug 2005 - Aug 2011
Research Assistant
Wagner College 2003 - 2005
Undergraduate Research Assistant
Columbia University In the City of New York 2004 - 2004
Undergraduate Research Assistant
Education:
Stony Brook University 2005 - 2011
Doctorates, Doctor of Philosophy, Biology, Philosophy, Chemistry Wagner College 2001 - 2005
Bachelors, Bachelor of Science, Chemistry Mahopac High School 2001
Skills:
Protein Chemistry
Chemical Biology
Biochemistry
Protein Purification
Pcr
Enzymes
Molecular Biology
Protein Expression
Drug Discovery
Peptides
Laboratory
Molecular Cloning
Western Blotting
Assay Development
Microbiology
Purification
Enzyme Kinetics
Protein Characterization
Janine Borgaro Photo 2

Janine G. Borgaro Beverly, MA

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Work:
New England Biolabs

2011 to 2000
Postdoctoral Research Fellow
Stony Brook University
New York, NY
2005 to 2011
Graduate Research Assistant
Wagner College
New York, NY
2003 to 2005
Undergraduate Research Assistant
Columbia University
New York, NY
Jun 2004 to Aug 2004
Undergraduate Research Assistant
Education:
Stony Brook University
2005 to 2011
Doctor of Philosophy in Chemistry
Stony Brook University
Stony Brook, NY
2001 to 2005
Bachelor of Science in Chemistry
Wagner College
New York, NY
Skills:
Molecular Biology: Performed plasmid construction, cloning recombinant DNA, site-directed mutagenesis, RT-PCR and inverse PCR Recombinant. Protein Expression: Many years of experience with culturing bacteria, protein expression and purification, affinity chromatography, gel filtration, ion exchange, FPLC, AKTA, dialysis, SDS or Native PAGE and western blotting. Next Generation Sequencing: Executed restriction enzyme based DNA library preparation for Illumina GAII and Ion Torrent sequencers. Biophysical Characterization: Expertise in mechanistic enzymology, Utilized fluorescence spectroscopy, UV-spectroscopy, Circular Dichroism (CD), Biacore, HPLC, ESI-MS, and LC-MS. Radiochemistry: Designed and implemented experiments using radioactive chemicals 11C, 14C, 3H, Quantitated radioactivity in instruments such as scintillation counters and phosphorimagers. Organic/Peptide Synthesis: Carried out the synthesis, purification and characterization of enzymatic substrates and small molecule probes (ESI-MS). Computer Proficiency: PyMOL (protein structure), Grafit it (Data processing), ChemDraw, CorelDraw.

Publications

Us Patents

Compositions And Methods For Identifying Hydroxymethylcytosine In A Dna

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US Patent:
20150004596, Jan 1, 2015
Filed:
Jun 27, 2014
Appl. No.:
14/317143
Inventors:
- Ipswich MA, US
Janine Borgaro - Beverly MA, US
Aine Quimby - Newton MA, US
Shengxi Guan - Stoneham MA, US
Zhiyi Sun - Gloucester MA, US
International Classification:
C12Q 1/68
C12N 9/22
US Classification:
435 61, 435196, 536 232, 4353201, 4352523, 506 11
Abstract:
Provided herein in some embodiments is a non-naturally occurring variant of a wild type restriction enzyme defined by SEQ ID NO: 20, wherein the variant has at least a 2 fold increase in cleavage at 5-β glucosylhydroxymethylcytosine (5βghmC) compared with methylcytosine relative to the wild type enzyme. Methods for examining hydroxymethylation of a DNA sample using the variant enzyme are also provided.

Mapping Cytosine Modifications

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US Patent:
20140127678, May 8, 2014
Filed:
Mar 14, 2013
Appl. No.:
13/826395
Inventors:
- Ipswich MA, US
Nan Dai - Gloucester MA, US
Zhenyu Zhu - Beverly MA, US
Aine Quimby - Newton NH, US
Janine Borgaro - Beverly MA, US
International Classification:
C12Q 1/68
US Classification:
435 61, 435 915, 435193, 435189
Abstract:
Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
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Janine G Borgaro from Winchester, MA, age ~41 Get Report