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James A Rybski

from Tucson, AZ
Age ~71
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Also known as:
James Dr Rybski, James Andrew Rybski, James A Ryski, Jim Rybski
Phone and address:
1880 Andromeda Pl, Tucson, AZ 85737
(520) 219-1321
Related to:
Nancy Rybski, 53Jeanne C SatterthwaiteTricia Croyle, 77Eugene RybskiDavida E Rybski
Connected to:
Brett D Rybski, 33Adam D Ryburn, 36Anthony J Ryburn, 38Jeff A Ryburn, 63Robert D Ryburn, 72Ronald E Ryburn, 54Ryan J Ryburn, 36Timmothy R Ryburn, 63Larissa Rybus, 39Dorian A Ryce, 37

James Rybski Phones & Addresses

  • 1880 Andromeda Pl, Tucson, AZ 85737 (520) 219-1321
  • Oro Valley, AZ
  • Algoma, WI
  • 24646 Via Carissa, Laguna Niguel, CA 92677
  • 28245 La Plumosa, Laguna Niguel, CA 92677
  • Black River Falls, WI
  • Wausau, WI
  • Lisle, IL
  • Dekalb, IL
  • Dana Point, CA
  • Orange, CA

Publications

Us Patents

Immunohistochemical Staining Method And Reagents Therefor

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US Patent:
52253252, Jul 6, 1993
Filed:
Mar 2, 1990
Appl. No.:
7/488348
Inventors:
Phillip C. Miller - Tucson AZ
Michael J. Degroff - Tucson AZ
Michael J. Gizinski - Tucson AZ
James A. Rybski - Tucson AZ
Pamela S. Vandivort - Tucson AZ
Assignee:
Ventana Medical Systems, Inc. - Tucson AZ
International Classification:
C12Q 168
G01N 128
G01N 3348
G01N 3353
US Classification:
435 6
Abstract:
The present invention provides an immunohistochemical staining method using improved reagents. In one aspect, the method uses an evaporation inhibitor liquid to prevent evaporation of reagents applied to an assay region of a slide.

Immunohistochemical Staining Method And Reagents Therefor

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US Patent:
53227719, Jun 21, 1994
Filed:
Aug 31, 1992
Appl. No.:
7/924053
Inventors:
James A. Rybski - Tucson AZ
Pamela S. Vandivort - Tucson AZ
Anthony L. Hartman - Tucson AZ
Phillip C. Miller - Tucson AZ
Michael J. Degroff - Tucson AZ
Michael J. Gizinski - Tucson AZ
Assignee:
Ventana Medical Systems, Inc - Tucson AZ
International Classification:
C12N 996
C12Q 137
G01N 134
G01N 3353
US Classification:
435 72
Abstract:
The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding.

Immunohistochemical Staining Method And Reagents Therefor

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US Patent:
54181380, May 23, 1995
Filed:
Mar 11, 1994
Appl. No.:
8/212415
Inventors:
Phillip C. Miller - Tucson AZ
Michael J. Degroff - Tucson AZ
Michael J. Gizinski - Tucson AZ
James A. Rybski - Tucson AZ
Pamela S. Vandivort - Tucson AZ
Anthony L. Hartman - Tucson AZ
Assignee:
Ventana Medical Systems, Inc. - Tucson AZ
International Classification:
G01N 33535
US Classification:
435 72
Abstract:
The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section)is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding.
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James A Rybski from Tucson, AZ, age ~71 Get Report