Inventors:
- Oakland CA, US
- Shoham, IL
Michael John McCarthy - Davis CA, US
Tina Jeoh Zicari - Davis CA, US
Emilio Javier Tozzi - West Chester OH, US
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA
ASPECT AI LTD. - Shoham
International Classification:
C12Q 3/00
C12P 19/02
C12P 19/14
G01N 11/04
G01N 24/08
Abstract:
The present disclosure is generally related a method for the liquefaction of high-solids biomass substrates. Particularly, biomass can be added to a reactor until a pressure drop, measured inline, reaches the maximum system limitations. A commercial enzyme mixture (specific for the particular type of biomass to be processed) may then be added to the biomass, forming a slurry. The pressure may be continuously monitored and when the pressure drop reaches a steady state (which can be determined by little or no change in pressure drop for several minutes), more biomass may then be added until the high pressure limit of the pump system is reached again. The method can be repeated until the desired quantity of biomass is processed.