US Patent:
20200199573, Jun 25, 2020
Inventors:
- North Andover MA, US
DAI SHI - Boxford MA, US
International Classification:
C12N 15/10
Abstract:
The invention purifies desired polynucleotide samples by removing impurities resulting from biochemical reactions or physical manipulation, such as excess oligonucleotides, salt and protein after a polymerase chain reaction (PCR), or by selecting polynucleotide of desired sizes from a mixture of polynucleotides of different sizes resulting from polynucleotide fragmentation procedures. Desired polynucleotides bind reversibly to a solid surface such as magnetic micro-particles whose surfaces are coated with or without a functional group, such as a carboxyl group, during the removal process of impurity or polynucleotides of unintended sizes. The polynucleotides can be DNA, RNA or polyamide nucleic acids (PNAs). As a result, polynucleotides bound to the solid surface are purified or selected for desired sizes.The present invention utilizes polyalkylene glycol of certain concentrations as removal method of impurity or polynucleotide of un-desirable sizes instead of using ethanol or isopropanol of certain concentrations, such as 70% or 80%, which have been used as standard method of impurity removal for the past 20 years.
