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Claudia Chiesa Phones & Addresses

  • 29703 Milo Dr, Marana, AZ 85653 (520) 682-9730
  • 35254 Saguaro Vista Dr, Marana, AZ 85653 (520) 682-9730
  • 2123 Edgewood Rd, Redwood City, CA 94062 (650) 261-9188
  • Emerald Hills, CA
  • Woodland, CA
  • Helendale, CA
  • Apple Valley, CA
  • Yolo, CA
  • Foster City, CA
  • 29703 E Milo Dr, Marana, AZ 85658

Emails

Publications

Us Patents

Multiplex Polynucleotide Capture Methods And Compositions

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US Patent:
6514699, Feb 4, 2003
Filed:
Jun 13, 2000
Appl. No.:
09/593312
Inventors:
Roger A. ONeill - San Carlos CA
Claudia Chiesa - Foster City CA
George Fry - San Carlos CA
Assignee:
PE Corporation (NY) - Foster City CA
International Classification:
C12Q 168
US Classification:
435 6, 536 231, 536 243
Abstract:
The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i. e. , an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i. e. , purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i. e. , the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution.

Method And Apparatus For Flow-Through Hybridization

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US Patent:
6638760, Oct 28, 2003
Filed:
Dec 3, 1998
Appl. No.:
09/204865
Inventors:
Claudia Chiesa - Redwood City CA
George A. Fry - San Carlos CA
Vergine C. Furniss - San Mateo CA
Stephen M. Lambert - Castro Valley CA
Roger ONeill - San Carlos CA
Majid Mehrpouyan - San Jose CA
Assignee:
PE Corporation (NY) - Norwalk CO
International Classification:
C12M 134
US Classification:
4352872, 436 6, 436 911, 4362871, 536 231, 536 243, 536 2433
Abstract:
The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.

Strand Displacement Methods Employing Competitor Oligonucleotides For Isolating One Strand Of A Double-Stranded Nucleic Acid

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US Patent:
6787310, Sep 7, 2004
Filed:
Jul 18, 2001
Appl. No.:
09/909001
Inventors:
Claudia Chiesa - Redwood City CA
Gary P. Schroth - San Ramon CA
Michael Egholm - Woodbridge CT
Assignee:
Applera Corporation - Foster City CA
International Classification:
C12Q 168
US Classification:
435 6, 436 94, 536 254
Abstract:
The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.

Electrophoretic Nucleic Acid Purification Method

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US Patent:
6827830, Dec 7, 2004
Filed:
Sep 20, 2000
Appl. No.:
09/666905
Inventors:
Gary Slater - Ottawa, CA
J. William Efcavitch - San Mateo CA
Guy Drouin - Gatineau, CA
Pascal Mayer - Eloise, FR
Jean Rousseau - Quebec, CA
Hong Yan Zhou - Ottawa, CA
Claudia Chiesa - Foster City CA
Robert Ruhfel - San Francisco CA
Roger ONeill - San Carlos CA
Assignee:
Applera Corporation - Foster City CA
University of Ottawa - Ottawa
International Classification:
G01N 27447
US Classification:
204457
Abstract:
An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.

Multiplex Polynucleotide Capture Methods And Compositions

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US Patent:
61240923, Sep 26, 2000
Filed:
Jun 12, 1997
Appl. No.:
8/873437
Inventors:
Roger A. O'Neill - San Carlos CA
Claudia Chiesa - Foster City CA
George Fry - San Carlos CA
Assignee:
The Perkin-Elmer Corporation - Foster City CA
International Classification:
C12Q 168
C12P 1934
C07H 2102
C07H 2104
US Classification:
435 6
Abstract:
The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i. e. , an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i. e. , purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i. e. , the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution.

Method And Silicate Composition For Conditioning Silica Surfaces

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US Patent:
62970097, Oct 2, 2001
Filed:
Jan 9, 1998
Appl. No.:
9/005075
Inventors:
David M. Demorest - Soquel CA
Stephen E. Moring - Lawrence KS
Claudia Chiesa - Redwood City CA
Assignee:
Perkin-Elmer Corporation - Foster City CA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li. sup. +, Na. sup. +, or K. sup. +. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.

Electrophoretic Nucleic Acid Purification Method

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US Patent:
61465119, Nov 14, 2000
Filed:
Jan 30, 1998
Appl. No.:
9/016531
Inventors:
Gary Slater - Ottawa, CA
J. William Efcavitch - San Mateo CA
Guy Drouin - Gatineau, CA
Pascal Mayer - Eloise, FR
Jean Rousseau - Quebec, CA
Hong Yan Zhou - Ottawa, CA
Claudia Chiesa - Foster City CA
Robert Ruhfel - San Francisco CA
Roger O'Neill - San Carlos CA
Assignee:
The Perkin-Elmer Corporation - Foster City CA
The University of Ottawa - Ottawa
International Classification:
G01N 2726
US Classification:
204457
Abstract:
An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well. According to the method, the first and second electrophoresis steps are effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.
Claudia Chiesa from Marana, AZ, age ~60 Get Report