Inventors:
R. Bruce Wallace - Greenbrae CA
Bijay K. Pal - Arcadia CA
Luis A. Ugozzoli - San Rafael CA
Dan Y. Wu - Bellevue WA
International Classification:
C12P 1934
C12Q 168
Abstract:
A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one allele and a mismatch with the other allele. This method allows the direct detection of the normal or the sickle cell. beta. -globin allele in genomic DNA without the additional steps of probe hybridization, ligation or restriction enzyme cleavage.