US Patent:
20080280303, Nov 13, 2008
Inventors:
Ethan S. Burstein - San Diego CA, US
Fabrice Piu - San Diego CA, US
Jian-Nong Ma - San Diego CA, US
Jacques Weissman - Carlsbad CA, US
David M. Weiner - San Diego CA, US
Audra L. Scully - Carlsbad CA, US
Norman Nash - San Diego CA, US
Tracy Spalding - San Diego CA, US
Erika Currier - San Diego CA, US
International Classification:
C12Q 1/68
G01N 33/53
Abstract:
Disclosed herein are methods for enabling or improving functional assays of G-protein coupled receptors through the use of co-expression of helper genes. In some cases, chimeras linking the regulatory domain of the rap1B protein to the effector region of the ras oncogene are used in conduction with existing functional assays for cellular proliferation. Furthermore, overexpression of other genes can further augment the enabling properties of ras/rap chimeras.