Argiris S Efstratiadis

20020099194, Jul 25, 2002

Jul 13, 2001

09/905311

Argiris Efstratiadis - Englewood NJ, US
Thomas Ludwig - New York NY, US
Ana Kljuic - New York NY, US
Katerina Politi - NYC NY, US

The Trustees of Columbia University - New York NY

A01K067/027
C07H021/04
C12N015/00

536/023200, 435/320100, 800/014000

The present invention provides a nucleic acid molecule comprising: (a) a region of DNA which is homologous to a region of an endogenous gene present in a genome of a cell of interest; (b) a first nucleic acid encoding an encephalomyocarditis internal ribosome entry site (EMCV IRES); (c) a second nucleic acid encoding a selectable marker which can be excised from the nucleic acid molecule if the nucleic acid molecule has been integrated into the genome of the cell of interest; and (d) a third nucleic acid encoding a gene of interest. The cell may be an animal cell, a yeast cell or a plant cell. The invention also provides for transgenic non-human animals which are created using the above described construct. The invention also provides methods for making such transgenic animals.

56376855, Jun 10, 1997

Jun 6, 1995

8/465811

Marcelo B. Soares - New York NY
Argiris Efstratiadis - Englewood NJ

The Trustees of Columbia University in the City of New York - New York NY

C07H 2104

536 231

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

44119941, Oct 25, 1983

Jun 8, 1978

5/913533

Walter Gilbert - Cambridge MA
Stephanie A. Broome - Cambridge MA
Lydia J. Villa-Komaroff - Boston MA
Argiris A. Efstratiadis - Cambridge MA

The President and Fellows of Harvard College - Cambridge MA

C12P 2104
C12P 2100
C12N 1500
C12N 100

435 71

A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

58306625, Nov 3, 1998

Jun 21, 1996

8/619542

Marcelo B. Soares - New York NY
Argiris Efstratiadis - Englewood NJ

The Trustees of Columbia University in the City of New York - New York NY

C12Q 168
C12P 1934
C07H 2104

435 6

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

54828457, Jan 9, 1996

Sep 24, 1993

8/126594

Marcelo B. Soares - New York NY
Argiris Efstratiadis - Englewood NJ

The Trustees of Columbia University in the City of New York - New York NY

C12P 1934

435 911

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

45657858, Jan 21, 1986

Aug 1, 1983

6/519303

Walter Gilbert - Cambridge MA
Stephanie A. Broome - Cambridge MA
Lydia J. Villa-Komaroff - Boston MA
Argiris A. Efstratiadis - Cambridge MA

The President and Fellows of Harvard College - Cambridge MA

C12N 100
C12N 1500
C12N 986
C07H 1512

435317

A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.