Inventors:
Aaron DiAntonio - Olivette MO, US
Bradley R. Miller - St. Louis MO, US
Jeffrey D. Milbrandt - Clayton MO, US
Craig A. Press - St. Louis MO, US
Assignee:
WASHINGTON UNIVERSITY - St. Louis MO
International Classification:
A61K 31/7088
A61K 31/415
C12Q 1/48
Abstract:
Methods of reducing Wallerian degeneration are disclosed. These methods comprise inhibiting expression or activity of a mixed lineage kinas such as a dual leucine-zipper-bearing kinase (DLK), inhibiting expression or activity of a molecule acting downstream from DLK, such as a c-Jun N-terminal kinase (JNK), or a combination thereof. Further disclosed are methods of screening candidate compounds for DLK inhibition activity. These methods comprise providing a neuronal culture comprising a plurality of axons; contacting the culture with a candidate compound and with an axon degeneration-triggering agent; and comparing axonal degeneration in the culture to a control culture comprising the axon degeneration-triggering agent but not the candidate compound.